Speedy™ Human LIF One-Step ELISA Kit
Sensitivity
10.3 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : SE50013
Synonyms
Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor
Validation Data Gallery
Product Information
| Product name | Speedy™ Human LIF One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 10.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3976 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 90% | 82%-96% |
| Cell culture supernatant | 92% | 80%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,138.6 | 39.8 | 3.5 |
| 2 | 8 | 294.2 | 5.8 | 2.0 |
| 3 | 8 | 143.8 | 5.8 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,148.9 | 34.8 | 3.0 |
| 2 | 16 | 297.1 | 14.2 | 4.8 |
| 3 | 16 | 145.9 | 8.0 | 5.5 |
Background Information
Leukemia inhibitory factor (LIF) is a multi-functional cytokine of the interleukin-6 (IL-6) superfamily. It utilises a receptor that consists of the LIF receptor β and gp130 and this receptor complex is also used by ciliary neurotrophic growth factor (CNTF), oncostatin M, cardiotrophin1 (CT1) and cardiotrophin-like cytokine (CLC). Functionally, LIF has been implicated in a many physiological processes including the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor |