AuthentiKine™ Human LIF ELISA Kit0 Publications

カタログ番号: KE00208

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
AuthentiKine™ Human LIF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture superntants

アッセイ方法:
Sandwich

感度:
0.4 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 80% 75%-85%
Cell culture superntants 85% 76%-96%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 171.7 7.24 4.2
2 20 332.8 14.31 4.3
3 20 711.2 38.59 5.4

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 160.8 4.99 3.1
2 24 329.5 11.83 3.6
3 24 696.0 29.38 4.2

製品概要
KE00208 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LIF ELISA kit is to be used to detect and quantify protein levels of endogenous LIF. The assay recognizes human LIF. An antibody specific for LIF has been pre-coated onto the microwells. The LIF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for LIF is added to detect the captured LIF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI
背景説明

Leukemia inhibitory factor (LIF) is a multi-functional cytokine of the interleukin-6 (IL-6) superfamily. It utilises a receptor that consists of the LIF receptor β and gp130 and this receptor complex is also used by ciliary neurotrophic growth factor (CNTF), oncostatin M, cardiotrophin1 (CT1) and cardiotrophin-like cytokine (CLC). Functionally, LIF has been implicated in a many physiological processes including the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.


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