Speedy™ Human LEPR One-Step ELISA Kit
Sensitivity
0.10 ng/mL
Range
0.47-30 ng/mL
Reactivity
Human
Cat no : SE50072
Synonyms
CD295, DB, HuB219, LEP R, LEP-R
Validation Data Gallery
Product Information
| Product name | Speedy™ Human LEPR One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.10 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3953 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 113% | 95%-120% |
| Cell culture supernatant | 121% | 116%-125% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 15.27 | 0.37 | 2.42 |
| 2 | 8 | 3.84 | 0.08 | 2.08 |
| 3 | 8 | 1.08 | 0.03 | 2.78 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 15.36 | 0.35 | 2.28 |
| 2 | 16 | 3.92 | 0.19 | 4.85 |
| 3 | 16 | 1.08 | 0.03 | 2.78 |
Background Information
The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction . Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3. LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD295, DB, HuB219, LEP R, LEP-R |