Recombinant Human LAG-3/CD223protein (Myc Tag, His Tag)
Species
Human
Purity
>85 %, SDS-PAGE
Tag
Myc Tag, His Tag
Activity
not tested
Cat no : Eg31550
Validation Data Gallery
Product Information
| Purity | >85 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human LAG-3 protein Leu23-Leu450 (Accession# P18627-1) with a Myc tag and a His tag at the C-terminus. |
| GeneID | 3902 |
| Accession | P18627-1 |
| PredictedSize | 51.8 kDa |
| SDS-PAGE | 58-68 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function.
References:
1. Workman, Creg J, and Dario A A Vignali. Journal of immunology (Baltimore, Md. : 1950) vol. 174,2 (2005): 688-95. 2. Goldberg, Monica V, and Charles G Drake. Current topics in microbiology and immunology vol. 344 (2011): 269-78. 3. Zhang, Qianxia et al. Science immunology vol. 2,9 (2017): eaah4569.