Human LAG-3/CD223 ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00997
Synonyms
CD223, LAG3, FDC, LAG 3, LAG-3
Validation Data Gallery
Product Information
KE00997 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LAG-3/CD223 ELISA kit is to be used to detect and quantify protein levels of endogenous human LAG-3/CD223. The assay recognizes human LAG-3/CD223. An antibody specific for human LAG-3/CD223 has been pre-coated onto the microwells. The human LAG-3/CD223 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human LAG-3/CD223 is added to detect the captured human LAG-3/CD223 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LAG-3/CD223 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3902 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 88%-97% |
| Cell lysate | 89% | 84%-94% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.27 | 0.36 | 6.92 |
| 2 | 8 | 1.26 | 0.06 | 5.16 |
| 3 | 8 | 0.70 | 0.05 | 7.52 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.81 | 0.59 | 12.20 |
| 2 | 16 | 1.23 | 0.13 | 10.17 |
| 3 | 16 | 0.66 | 0.08 | 12.26 |
Background Information
LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. Additionally, LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD223, LAG3, FDC, LAG 3, LAG-3 |