Human Integrin beta-6 ELISA Kit
Sensitivity
0.08 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Cat no : KE00856
Synonyms
ITGB6, Integrin beta 6, integrin, beta 6
Validation Data Gallery
Product Information
KE00856 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Integrin beta-6 ELISA kit is to be used to detect and quantify protein levels of endogenous human Integrin beta-6. The assay recognizes human Integrin beta-6. An antibody specific for human Integrin beta-6 has been pre-coated onto the microwells. The human Integrin beta-6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Integrin beta-6 is added to detect the captured human Integrin beta-6 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Integrin beta-6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.08 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3694 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 103% | 91%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 19.95 | 1.34 | 6.70 |
| 2 | 8 | 4.90 | 0.09 | 1.84 |
| 3 | 8 | 2.52 | 0.05 | 1.96 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 19.95 | 1.29 | 6.47 |
| 2 | 16 | 4.97 | 0.15 | 3.04 |
| 3 | 16 | 2.64 | 0.13 | 5.09 |
Background Information
Integrin beta-6 (ITGB6) is a key submonomer of integrin αvβ6. It is exclusively expressed on surfaces of epithelial cells, upregulated during the epithelial-to-mesenchymal transition (EMT), and is involved in wound healing, epithelial-derived tumor growth, fibrosis, and epithelial repair. ITGB6 was found to be the key activator of TGFβ1 in lung epithelial cells. TGFβ1 could promote the expression of SMYD3 and ITGB6 via feedback. This positive feedback loop could further amplify the biological effect and promote the invasion and adhesion of ovarian cancer spheroids. Additionally, ITGB6 in serum might serve as a novel tumor marker for colorectal cancer patients.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ITGB6, Integrin beta 6, integrin, beta 6 |