Speedy™ Human IgG4 One-Step ELISA Kit
Sensitivity
2.5 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : SE50304
Synonyms
human IgG heavy chain, Human IgG4, IgG Fc, IGHG, IGHG4
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IgG4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 2.5 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3503 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 89%-108% |
| Urine | 100% | 95%-107% |
| Saliva | 94% | 92%-97% |
| Human milk | 90% | 75%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,629.7 | 77.3 | 2.9 |
| 2 | 8 | 670.0 | 15.0 | 2.2 |
| 3 | 8 | 335.0 | 8.8 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,665.3 | 83.0 | 3.1 |
| 2 | 16 | 659.4 | 19.8 | 3.0 |
| 3 | 16 | 338.4 | 9.8 | 2.9 |
Background Information
Immunoglobulin G4 (IgG4) is the least abundant subclass of immunoglobulin G, comprising only 3-6% of total serum IgG. It exhibits unique structural properties, including Fab-arm exchange and asymmetric half-molecules, resulting in monovalent binding and anti-inflammatory functions. Unlike other IgG subclasses, IgG4 does not effectively activate complement or engage Fc receptors, classifying it as a "blocking antibody" that can dampen immune responses. Clinically, elevated IgG4 levels are pathognomonic for IgG4-related disease (IgG4-RD), a fibroinflammatory condition characterized by tumefactive lesions, dense lymphoplasmacytic infiltrates rich in IgG4-positive plasma cells, and storiform fibrosis. First recognized in autoimmune pancreatitis, IgG4-RD now encompasses multiple organ systems including the biliary tract, retroperitoneum, salivary glands, and kidneys.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | human IgG heavy chain, Human IgG4, IgG Fc, IGHG, IGHG4 |