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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IgG3 Rabbit Recombinant Antibody (98724-1-RR)

    FC experiment of human PBMCs using 98724-1-RR (same clone as 98724-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IgG3 Rabbit RecAb (98724-1-RR, Clone: 251300H6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). The cells originate from CD11b-negative cells. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98724-1-PBS in a different storage buffer formulation.

  • Affinity of 98724-1-RR

    Affinity of 98724-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98724-1-RR against Human IgG3 were performed. The affinity constant is 1.92 nM.

Anti-Human IgG3 Rabbit Recombinant Antibody, PBS Only

IgG3 Uni-rAb® Recombinant Antibody for FC
Cat No. 98724-1-PBS
Clone No.251300H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IGHG3

Formulation:  PBS Only
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98724-1-PBS targets IgG3 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6674

Product name: recombinant human IgG3-IgK protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10009500 of

Sequence: /

相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant gamma 3 (G3m marker)
Gene Symbol IGHG3
Gene ID (NCBI) 3502
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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