Validation Data Gallery
Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98804-3-RR targets IgG1 in IHC, FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6672 Product name: recombinant human IgG1-IgK protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Sequence: / 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Gene Symbol | IGHG1 |
| Gene ID (NCBI) | 3500 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
Human Immunoglobulin G subclass 1 (IgG1) is the most abundant antibody subclass in human serum, constituting approximately 60-70% of total IgG. It serves as a critical mediator of the adaptive immune system's defense against pathogens. Human IgG1 represents a perfect balance of specificity, stability, and powerful immune-activating capacity. Its dominance in the biotherapeutic landscape is a testament to its versatility and effectiveness. This antibody does not recognize IgA2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG1 antibody 98804-3-RR | Download protocol |
| IHC protocol for IgG1 antibody 98804-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |