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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Human Ig lambda chain Monoclonal antibody (67762-1-Ig)

    WB analysis of human plasma using 67762-1-Ig (same clone as 67762-1-PBS)

    80 nL human plasma were subjected to SDS PAGE followed by western blot with 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human Ig lambda chain Monoclonal antibody (67762-1-Ig)

    IHC staining of human tonsillitis using 67762-1-Ig (same clone as 67762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human Ig lambda chain Monoclonal antibody (67762-1-Ig)

    IHC staining of human tonsillitis using 67762-1-Ig (same clone as 67762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human Ig lambda chain Monoclonal antibody (67762-1-Ig)

    IHC staining of human tonsillitis using 67762-1-Ig (same clone as 67762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using Human Ig lambda chain Monoclonal antibody (67762-1-Ig)

    IF Staining of human appendicitis using 67762-1-Ig (same clone as 67762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG lambda chain antibody (67762-1-Ig, Clone: 1D12B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67762-1-PBS in a different storage buffer formulation.

Human Ig lambda chain Monoclonal antibody, PBS Only

Human Ig lambda chain Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67762-1-PBS
Clone No.1D12B5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Human IgG lambda chain, IgG Lambda Light Chain, IGLC1, 1D12B5, IGLC

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67762-1-PBS targets Human Ig lambda chain in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag14783

Product name: Recombinant human IGLC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-234 aa of BC007782

Sequence: MAWTVLLLGLLSHCTGSGTSYVLTQPASVSVAPGQTARITCGGSNLGSKSVNWYQLRPGQAPILVVYENKERPAGIPERLSALTSEETATLTISSVVAGDEADYFCQVWDTTSQQYVFGTGTQVTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS

相同性解析による交差性が予測される生物種
Full Name immunoglobulin lambda constant 1 (Mcg marker)
Calculated molecular weight 233 aa, 25 kDa
Observed molecular weight~25 kDa
GenBank accession numberBC007782
Gene Symbol IgG Lambda Light Chain
Gene ID (NCBI) 3537
RRIDAB_2918530
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

This antibody detects Lambda chain of human immunoglobulin.

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