Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma | 
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0  | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67761-1-Ig targets Human IgG Kappa chain in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human | 
| Host / Isotype | Mouse / IgG1 | 
| Class | Monoclonal | 
| Type | Antibody | 
| Immunogen | 
                                             Peptide 相同性解析による交差性が予測される生物種 | 
                                    
| Full Name | Human IgG Kappa chain | 
| Calculated molecular weight | 155 kDa | 
| Observed molecular weight | ~25 kDa | 
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918529 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Protein G purification | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Background Information
This antibody detects the Kappa chain of human IgG,
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Human IgG Kappa chain antibody 67761-1-Ig | Download protocol | 
| WB protocol for Human IgG Kappa chain antibody 67761-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 





