Human IgG Polyclonal antibody

Human IgG Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10284-1-AP

Synonyms

Human IgG



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

10284-1-AP targets Human IgG in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Human IgG
Calculated molecular weight 155 kDa
Observed molecular weight 25 kDa, 50 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_2877729
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This rabbit poly clonal antibody recognizes the human IgG.

Protocols

Product Specific Protocols
WB protocol for Human IgG antibody 10284-1-APDownload protocol
IHC protocol for Human IgG antibody 10284-1-APDownload protocol
IF protocol for Human IgG antibody 10284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Adv

Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer.

Authors - Long Qin

Int Immunopharmacol

LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals.

Authors - Zhi-Min Wang

Front Bioeng Biotechnol

Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.

Authors - Rui-Rui Guo

Biosensors (Basel)

Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber.

Authors - Yijian Huang
CoIP

Int J Oral Sci

FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.

Authors - Xinyuan Zhao
humanWB

Clin Exp Metastasis

Mass spectrometry-based proteomic capture of proteins bound to the MACC1 promoter in colon cancer.

Authors - Yahui Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Fatima (Verified Customer) (12-11-2018)

  • Applications: Flow Cytometry,