Validation Data Gallery
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Tested Applications
| Positive WB detected in | human plasma tissue |
| Positive IP detected in | human plasma |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10284-1-AP targets Rabbit Anti-Human IgG in WB, IHC, IF-P, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Rabbit Anti-Human IgG |
| Calculated molecular weight | 155 kDa |
| Observed molecular weight | 25 kDa, 50 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2877729 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This rabbit poly clonal antibody recognizes the human IgG.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Rabbit Anti-Human IgG antibody 10284-1-AP | Download protocol |
| IHC protocol for Rabbit Anti-Human IgG antibody 10284-1-AP | Download protocol |
| IP protocol for Rabbit Anti-Human IgG antibody 10284-1-AP | Download protocol |
| WB protocol for Rabbit Anti-Human IgG antibody 10284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Oncogenic fatty acid oxidation senses circadian disruption in sleep-deficiency-enhanced tumorigenesis | ||
Cancer Res Breast Cancer Stem Cells Secrete MIF to Mediate Tumor Metabolic Reprogramming that Drives Immune Evasion | ||
Drug Resist Updat Endothelial SMAD1-MCAM axis facilitates sunitinib resistance and progression of clear cell renal cell carcinoma via LAMB1-ITGB1 signaling. | ||
Nat Commun Sterically chained amino acid-rich water-soluble carbon quantum dots as a robust tumor-targeted drug delivery platform | ||
Int J Oral Sci FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1. | ||
J Exp Clin Cancer Res Histone acetyltransferase CSRP2BP promotes the epithelial-mesenchymal transition and metastasis of cervical cancer cells by activating N-cadherin |