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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Human INSL4 protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2884

Validation Data Gallery

  • Purity of Recombinant Human INSL4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human INSL4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human INSL4 was greater than 90% as determined by SEC-HPLC.

    The purity of Human INSL4 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human INSL4 protein Ala26-Thr139 (Accession# Q14641) with a rabbit IgG Fc tag at the C-terminus.
GeneID 3641
Accession Q14641
PredictedSize 38.6 kDa
SDS-PAGE 28-33 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The early placental insulin-like peptide, (EPIL or INSL4) is also a member of the insulin superfamily, it has the most homology with the human relaxins, RLN1 and RLN2 (44% and 43% respectively), and only 15% homology to insulin. It was initially identified from a subtracted cDNA library of a first trimester placenta, and was shown to be most highly expressed in embryonic and trophoblastic tissues.

References:

1. Chassin D, et al. (1995). Genomics. 29:465–470.
2. Lin F, et al. (2004). J Pept Sci. 10:257–264.
3. Koman A, et al. (1996). J Biol Chem. 271:20238–20241.
4. Laurent A, et al. (1998). Mol Reprod Dev. 51:123–129.