Human INO80 ELISA Kit
Sensitivity
7.7 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : KE00497
Synonyms
INO80,Chromatin-remodeling ATPase INO80,DNA helica
Validation Data Gallery
Product Information
KE00497 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human INO80 ELISA kit is to be used to detect and quantify protein levels of endogenous human INO80. The assay recognizes human INO80. An antibody specific for human INO80 has been pre-coated onto the microwells. The human INO80 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human INO80 is added to detect the captured human INO80 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human INO80 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 7.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54617 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 115% | 112%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,739.3 | 201.1 | 7.3 |
| 2 | 8 | 619.2 | 40.2 | 6.5 |
| 3 | 8 | 322.9 | 23.0 | 7.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,801.9 | 236.7 | 8.4 |
| 2 | 16 | 645.3 | 53.8 | 8.3 |
| 3 | 16 | 333.6 | 27.3 | 8.2 |
Background Information
INO80 is the main ATPase subunit of the INO80 complex, which has broad effects on several cellular processes. INO80 is important in various DNA metabolic processes, including DNA replication, transcription, repair, and genome stability in several organisms. The exchange and turnover of histone variant H2A.Z is regulated by INO80.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | INO80,Chromatin-remodeling ATPase INO80,DNA helica |