Human IL6R ELISA Kit0 Publications

カタログ番号: KE00118

51,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL6R ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants, Urine

アッセイ方法:
Sandwich

感度:
0.1 pg/mL

測定範囲:
7.8 - 500 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 89% 80%-119%
Cell culture supernatants 94% 82%-108%
Urine 97% 80%-108%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 236.9 8.2 3.5
2 20 61.6 1.8 2.9
3 20 15.1 0.5 3.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 271.4 12.9 4.8
2 24 67.2 3.2 4.8
3 24 16.0 0.8 5.2

製品概要
KE00118 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6R ELISA kit is to be used to detect and quantify protein levels of endogenous IL6R. The assay recognizes human IL6R. An antibody specific for IL6R has been pre-coated onto the microwells. The IL6R protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL6R is added to detect the captured IL6R protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL6R, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80
背景説明

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge. IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor. Expression of gp130 is found in almost all organs while expression of IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells). Soluble form of IL-6R has been found in human serum and urine.


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