Human IL-2RA ELISA Kit0 Publications

カタログ番号: KE00140

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL-2RA ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
5.8 pg/mL

測定範囲:
78.1-5000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 92% 70%-125%
Cell culture supernatants 101% 86%-113%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 2,100.3 208.2 9.9
2 20 553.1 48.6 8.8
3 20 129.1 11.1 8.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 2,486.2 237.1 9.5
2 24 616.5 48.4 7.9
3 24 136.2 12.4 9.1

製品概要
KE00140 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL2RA ELISA kit is to be used to detect and quantify protein levels of endogenous IL2RA. The assay recognizes human IL2RA. An antibody specific for IL2RA has been pre-coated onto the microwells. The IL2RA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human IL2RA is added to detect the captured human IL2RA protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR
背景説明

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. Soluble IL-2R alpha has been isolated and determined to result from extracellular proteolyisis. Soluble IL-2R alpha has been found in the plasma of patients with certain hematologic malignancies. Elevated plasma or serum concentration of soluble IL-2R alpha has also been reported as a biomarker of immune activation.


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