Recombinant Human IL12A protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3341
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human IL12A protein Arg23-Ser219 (Accession# P29459) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 3592 |
| Accession | P29459 |
| PredictedSize | 48.8 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin-12A (IL-12A), also known as p35, is a protein that plays a crucial role in the immune system. It is a subunit of the cytokine IL-12, which is a heterodimeric protein composed of two disulfide-linked subunits: IL-12A (p35) and IL-12B (p40). IL-12A is encoded by the IL12A gene located on chromosome 3 and is expressed in various cell types, including professional antigen-presenting cells such as B-cells, dendritic cells, macrophages, and granulocytes. The primary function of IL-12A is to regulate T-cell and natural killer-cell responses. It is particularly important for the production of interferon-gamma (IFN-gamma), the differentiation of T-helper 1 (Th1) cells, and serves as a link between innate resistance and adaptive immunity.
References:
1.Wolf SF. et al. (1991) J Immunol. 146(9):3074-81. 2.Gubler U. et al. (1991) Proc Natl Acad Sci U S A. 88(10):4143-7. 3.Ullrich KA. et al. (2020) EXCLI J. 19:1563-1589.