AuthentiKine™ Human IL-10 ELISA Kit
Cat no : KE00170
Synonyms
IL-10,IL10, CSIF, IL 10, IL10, IL10A
Validation Data Gallery
Product Information
KE00170 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous IL10. The assay recognizes human IL10. An antibody specific for IL10 has been pre-coated onto the microwells. The IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL10 is added to detect the captured IL10 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-10 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3586 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| human plasma | 92% | 79%-100% |
| Cell culture supernatants | 102% | 88%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 67.8 | 4.5 | 6.7 |
| 2 | 20 | 175.0 | 10.0 | 5.7 |
| 3 | 20 | 926.2 | 42.2 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 193.9 | 17.6 | 9.1 |
| 2 | 24 | 432.4 | 38.9 | 9.0 |
| 3 | 24 | 1,108.4 | 111.3 | 10.0 |
Background Information
Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL-10,IL10, CSIF, IL 10, IL10, IL10A |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nanobiotechnology Hypoxic glioma-derived exosomal miR-25-3p promotes macrophage M2 polarization by activating the PI3K-AKT-mTOR signaling pathway | ||
Cell Death Dis Hypoxic glioma-derived exosomes promote M2-like macrophage polarization by enhancing autophagy induction. | ||
J Neuroinflammation Berberine ameliorates depression-like behaviors in mice via inhibiting NLRP3 inflammasome-mediated neuroinflammation and preventing neuroplasticity disruption | ||
Mol Ther Exosomal miR-1246 from glioma patient body fluids drives the differentiation and activation of myeloid-derived suppressor cells. | ||
Clin Cancer Res Hypoxia-induced neuronal activity in glioma patients polarizes microglia by potentiating RNA m6A demethylation | ||
Cell Death Discov Suppression of the hyaluronic acid pathway induces M1 macrophages polarization via STAT1 in glioblastoma. |