Speedy™ Human/Cynomolgus Monkey IL-4 One-Step ELISA Kit
Sensitivity
1.9 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human/Cynomolgus Monkey
Cat no : SE50018
Synonyms
IL4, B-cell stimulatory factor 1, BSF-1, IFN-beta-2, IL 4
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey IL-4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cynomolgus monkey serum, Cynomolgus monkey plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3565 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 86% | 80%-93% |
| Cell culture supernatant | 107% | 90%-116% |
| Cynomolgus monkey serum | 98% | 89%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,092.3 | 18.7 | 1.7 |
| 2 | 8 | 253.7 | 7.8 | 3.1 |
| 3 | 8 | 120.6 | 2.1 | 1.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,069.6 | 35.1 | 3.3 |
| 2 | 16 | 249.7 | 8.1 | 3.2 |
| 3 | 16 | 118.1 | 3.5 | 3.0 |
Background Information
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4 + T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL4, B-cell stimulatory factor 1, BSF-1, IFN-beta-2, IL 4 |