Speedy™ Human IL-1R1 One-Step ELISA Kit
Sensitivity
14.7 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : SE50052
Synonyms
IL1R1, CD121 antigen-like family member A, CD121A, D2S1473, EC:3.2.2.6
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IL-1R1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 14.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3554 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 94% | 91%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,579.3 | 57.0 | 2.2 |
| 2 | 8 | 673.0 | 10.9 | 1.6 |
| 3 | 8 | 193.4 | 5.1 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,586.0 | 63.6 | 2.5 |
| 2 | 16 | 674.9 | 13.7 | 2.0 |
| 3 | 16 | 192.2 | 8.0 | 4.2 |
Background Information
Interlukin-1 polypeptides are pleiotropic cytokines exerting diverse functions in different tissues. IL-1R1 (also known as CD121a) is a receptor for interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), and interleukin-1 receptor antagonist (IL-1Ra). Human IL-1R1 was first cloned from human T cells. This receptor contains a large cytoplasmic region and an extracellular, IL-1 binding portion composed of three immunoglobulin-like domains. IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be shed from the cell membrane as a soluble form (sIL-1R1) which can function as a soluble decoy receptor and prevent the binding of IL-1α, IL-1β, and IL-1Ra to membrane IL-1R1.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL1R1, CD121 antigen-like family member A, CD121A, D2S1473, EC:3.2.2.6 |