Human IL-1R1 ELISA Kit
Sensitivity
4.3 pg/mL
Range
39-2500 pg/mL
Reactivity
Human
Cat no : KE00939
Synonyms
IL1R1, CD121 antigen-like family member A, CD121A, D2S1473, EC:3.2.2.6
Validation Data Gallery
Product Information
KE00939 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IL-1R1 ELISA kit is to be used to detect and quantify protein levels of endogenous human IL-1R1. The assay recognizes human IL-1R1. An antibody specific for human IL-1R1 has been pre-coated onto the microwells. The human IL-1R1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IL-1R1 is added to detect the captured human IL-1R1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-1R1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 4.3 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3554 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 93%-104% |
| Cell lysate | 89% | 77%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,219.4 | 55.4 | 4.5 |
| 2 | 8 | 327.5 | 26.6 | 8.1 |
| 3 | 8 | 154.7 | 5.5 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,203.2 | 106.5 | 8.8 |
| 2 | 16 | 318.4 | 28.4 | 8.9 |
| 3 | 16 | 147.4 | 9.3 | 6.3 |
Background Information
Interlukin-1 polypeptides are pleiotropic cytokines exerting diverse functions in different tissues. IL-1R1 (also known as CD121a) is a receptor for interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), and interleukin-1 receptor antagonist (IL-1Ra). Human IL-1R1 was first cloned from human T cells. This receptor contains a large cytoplasmic region and an extracellular, IL-1 binding portion composed of three immunoglobulin-like domains. IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be shed from the cell membrane as a soluble form (sIL-1R1) which can function as a soluble decoy receptor and prevent the binding of IL-1α, IL-1β, and IL-1Ra to membrane IL-1R1.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL1R1, CD121 antigen-like family member A, CD121A, D2S1473, EC:3.2.2.6 |