Recombinant Human IL-15 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg1980
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human IL-15 protein Asn49-Ser162 (Accession# P40933-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 3600 |
| Accession | P40933-1 |
| PredictedSize | 39.1 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin 15 (IL15) is a cytokine important for the development, maturation, and function of many cells of the immune system including NK, NKT, gammadeltaT, and CD8(+) T cells. Its expression affects homeostasis and function of B cells through NK cell-derived IFNG. IL-15 binds a trimeric IL-15 receptor (IL-15R) complex formed by a specific receptor chain (IL-15Rα) combined with theβandγchains of the IL-2R, acting as signal transducing components. High levels of IL15 expression have been associated with the pathogenesis of autoimmune and inflammatory diseases like Crohn's disease, psoriasis, leukemias, rheumatoid arthritis (RA) and graft rejection, resulting in the IL15 system being a target of interest for the treatment of these diseases.
References:
1. Gill, Navkiran et al. Cellular immunology vol. 258,1 (2009): 59-64. 2. Kirman, I, and O H Nielsen. The American journal of gastroenterology vol. 91,9 (1996): 1789-94. 3. Rückert, R et al. Journal of immunology (Baltimore, Md. : 1950) vol. 165,4 (2000): 2240-50. 4. Yamada, Y, and S Kamihira. Leukemia & lymphoma vol. 35,1-2 (1999): 37-45. 5. Pavlakis, M et al. Transplantation vol. 62,4 (1996): 543-5. 6. Manfro, R C et al. Transplantation proceedings vol. 29,1-2 (1997): 1077-8.