Human IL-13 ELISA Kit
Sensitivity
1.0 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Cat no : KE01032
Synonyms
IL13, IL 13, interleukin 13, Interleukin-13, NC30
Validation Data Gallery
Product Information
KE01032 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-13 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-13. The assay recognizes human IL-13. An antibody specific for IL-13 has been pre-coated onto the microwells. The IL-13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-13 is added to detect the captured IL-13 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-13 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3596 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 77% | 72%-84% |
| Cell culture supernatant | 99% | 94%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,214.0 | 80.3 | 3.6 |
| 2 | 8 | 509.1 | 23.7 | 4.7 |
| 3 | 8 | 272.5 | 14.6 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,164.8 | 123.9 | 5.7 |
| 2 | 16 | 513.1 | 23.6 | 4.6 |
| 3 | 16 | 273.2 | 17.9 | 6.6 |
Background Information
Interleukin 13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. IL-13 up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. IL-13 is thought to be important cytokine in the pathogenesis of asthma, and more recently has been shown to play a pivotal role in a number of fibrotic diseases including hepatic and pulmonary fibrosis, and nodular sclerosing Hodgkin's disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL13, IL 13, interleukin 13, Interleukin-13, NC30 |