Recombinant Human IL-12 (linked heterodimer) protein (hFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
hFc Tag
Activity
not tested
Cat no : Eg0606
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human IL-12B Ile23-Ser328 (Accession# P29460) +linker+IL-12A Arg23-Ser219 (Accession# P29459) with a human IgG1 Fc tag at the C-terminus. |
| GeneID | 3593(IL-12B),3592(IL-12A) |
| Accession | P29460 (IL-12B), P29459 (IL-12A) |
| PredictedSize | 85.3 kDa |
| SDS-PAGE | 85-100 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine that induces the production of interferon-gamma (IFN-gamma), favours the differentiation of T helper 1 (T(H)1) cells and forms a link between innate resistance and adaptive immunity. It consists of two subunits, p35 and p40, which are encoded by IL12A and IL12B, respectively. Furthermore, IL-12 binds to the IL-12 receptor, which is a heterodimeric receptor formed by the IL-12R-β1 and IL-12R-β2 subunits.
References:
1.Trinchieri, Giorgio. (2003) Nat Rev Immunol.3(2):133-146. 2.Picard, Capucine et al. (2002) Am J Hum Genet.70(2):336-348. 3.Lin, Eugene et al. (2019) Front Neurol.10:1065.