Speedy™ Human IL-12/IL-23 p40 One-Step ELISA Kit
Sensitivity
2.3 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : SE50032
Synonyms
IL12B, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40, IL 12 subunit p40
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IL-12/IL-23 p40 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3593 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 83%-108% |
| Cell culture supernatant | 99% | 91%-103% |
| Urine | 99% | 93%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 497.0 | 6.3 | 1.3 |
| 2 | 8 | 124.1 | 2.5 | 2.0 |
| 3 | 8 | 62.7 | 1.2 | 1.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 490.4 | 11.6 | 2.4 |
| 2 | 16 | 123.0 | 2.8 | 2.3 |
| 3 | 16 | 61.4 | 1.8 | 2.9 |
Background Information
Interleukin-12 (IL-12) and IL-23 are cytokines that share a common subunit, p40. IL-12 is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen-presenting cells (APCs), including phagocytes, B cells and Langerhans cells. IL-12 p40 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of IL-12 p40 was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of IL-12 p40 has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL12B, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40, IL 12 subunit p40 |