Speedy™ Human/Cynomolgus Monkey IL-10 One-Step ELISA Kit
Sensitivity
3.5 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human/Cynomolgus Monkey
Cat no : SE50211
Synonyms
IL-10,IL10, CSIF, IL 10, IL10, IL10A
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey IL-10 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 3.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3586 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 81%-95% |
| Cell culture supernatant | 99% | 92%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 935.2 | 40.5 | 4.3 |
| 2 | 8 | 222.2 | 4.3 | 1.9 |
| 3 | 8 | 110.9 | 2.4 | 2.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 925.6 | 34.5 | 3.73 |
| 2 | 16 | 218.9 | 5.3 | 2.42 |
| 3 | 16 | 111.2 | 3.5 | 3.15 |
Background Information
Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL-10,IL10, CSIF, IL 10, IL10, IL10A |