Speedy™ Human IL-1 alpha One-Step ELISA Kit
Sensitivity
1.6 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Cat no : SE50028
Synonyms
IL 1 alpha, IL1A, Hematopoietin-1, IL 1A, IL1 ALPHA
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IL-1 alpha One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.6 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3552 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 90% | 81%-103% |
| Cell culture supernatant | 101% | 97%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 329.1 | 5.1 | 1.5 |
| 2 | 8 | 75.1 | 1.4 | 1.9 |
| 3 | 8 | 37.6 | 0.6 | 1.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 325.0 | 14.4 | 4.4 |
| 2 | 16 | 74.2 | 1.7 | 2.3 |
| 3 | 16 | 37.2 | 0.7 | 1.9 |
Background Information
Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL 1 alpha, IL1A, Hematopoietin-1, IL 1A, IL1 ALPHA |