Human IL-1 alpha ELISA Kit2 Publications

カタログ番号: KE00123

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL-1 alpha ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
0.7 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatants 101% 89%-110%
Human plasma 93% 74%-116%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 109.5 6.3 5.7
2 20 290.6 24.5 8.4
3 20 639.4 60.2 9.4

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 128.6 5.0 3.9
2 24 335.7 21.0 6.3
3 24 688.6 62.2 9.0

製品概要
KE00123 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1 alpha ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1 alpha. The assay recognizes human IL-1 alpha. An antibody specific for IL-1 alpha has been pre-coated onto the microwells. The IL-1 alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1 alpha is added to detect the captured IL-1 alpha protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
Hematopoietin 1, IL 1A, IL1, IL1 ALPHA, IL-1 alpha, IL1- alpha, IL1A, IL-1alpha, IL1F1, Interleukin 1 alpha, interleukin 1, alpha
背景説明

Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 alpha is a cytokine and a potent mediator of body’s response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer’s disease and tumor growth. IL-1 alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.


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