Speedy™ Human IGFBP3 One-Step ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : SE50073
Synonyms
BP 53, IBP 3, IBP3, IBP-3, IGF binding protein 3
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IGFBP3 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3486 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 97% | 88%-106% |
| Cell culture supernatant | 106% | 88%-116% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.65 | 0.09 | 1.94 |
| 2 | 8 | 1.17 | 0.05 | 4.27 |
| 3 | 8 | 0.31 | 0.01 | 3.23 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.81 | 0.20 | 4.16 |
| 2 | 16 | 1.24 | 0.10 | 8.06 |
| 3 | 16 | 0.34 | 0.03 | 8.82 |
Background Information
Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BP 53, IBP 3, IBP3, IBP-3, IGF binding protein 3 |