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Recombinant human IGF-I/IGF-1 protein

Species

Human

Purity

>90 %, SDS-PAGE

Tag

hFc Tag

Activity

EC50: 20-80 ng/mL

Cat no : Eg0206

Validation Data Gallery

  • Purity of Recombinant Human IGF1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IGF1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human IGF1 R (GST tag) at 1 μg/mL (100 μL/well) can bind Human IGF1 (hFc tag) with a linear range of 20-80 ng/mL.

    Immobilized Human IGF1 R (GST tag) at 1 μg/mL (100 μL/well) can bind Human IGF1 (hFc tag) with a linear range of 20-80 ng/mL.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human IGF1 R (GST tag) at 1 μg/mL (100 μL/well) can bind Human IGF1 (hFc tag) with a linear range of 20-80 ng/mL.
Expression HEK293-derived Human IGF1 protein Gly49-Ala118 (Accession# P05019-1) with a human IgG1 Fc tag at the N-terminus.
GeneID 3479
Accession P05019-1
PredictedSize 33.7 kDa
SDS-PAGE 34-36 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone is the principal mediator of biochemical effects of growth hormone (GH). IGF1 is an important growth factor in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 have been shown to play an essential role in preventing the formation of fatty liver. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas.

References:

1. Adamo ML. et al. (1993) Adv Exp Med Biol. 343:1-11.
2. Adachi Y. et al. (2019) J Gastroenterol Hepatol. 2019 Jun 3.
3. Anisimov VN. et al. (2019) Crit Rev Oncol Hematol. 87(3):201-23.
4. Sonntag WE. et al. (2012) J Gerontol A Biol Sci Med Sci. 67(6):587-98.
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