Recombinant Human IFNA5 protein (rFc Tag)(HPLC verified)
Species
Human
Purity
>90 %, SDS-PAGE
>90 %, SEC-HPLC
Tag
rFc Tag
Activity
not tested
Cat no : Eg4960
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human IFNA5 protein Leu22-Glu189 (Accession# P01569) with a rabbit IgG Fc tag at the N-terminus. |
| GeneID | 3442 |
| Accession | P01569 |
| PredictedSize | 46.9 KDa |
| SDS-PAGE | 45-50 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in 0.5M arg, 30mM Tris, pH8.0. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors. The mature human IFNA5, also known as IFN-alpha G, shares 40% aa sequence identity with mouse IFNA5. IFNA5 is the only IFNA subtype detected in normal liver and has been shown to induce stronger signaling and higher expression of antiviral genes than IFNA2 in hepatocytic cells.
References:
1. Pestka S. et al. (1987). Annu. Rev. Biochem. 56:727. 2. Larrea E. et al. (2004). J Interferon Cytokine Res. 24:497.