Speedy™ Human IDO1 One-Step ELISA Kit
Sensitivity
0.05 ng/mL
Range
0.39-25 ng/mL
Reactivity
Human
Cat no : SE50047
Synonyms
EC:1.13.11.52, IDO, IDO 1, IDO-1, INDO
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IDO1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants,Cerebrospinal fluid |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3620 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 91% | 79%-103% |
| Cell culture supernatant | 107% | 100%-111% |
| CSF | 104% | 98%-107% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 12.74 | 0.34 | 2.67 |
| 2 | 8 | 3.06 | 0.07 | 2.29 |
| 3 | 8 | 1.50 | 0.02 | 1.33 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 12.73 | 0.25 | 1.96 |
| 2 | 16 | 3.06 | 0.06 | 1.96 |
| 3 | 16 | 1.51 | 0.03 | 1.99 |
Background Information
Indoleamine 2, 3-dioxygenase 1 (IDO1) is a rate-limiting metabolic enzyme that converts the essential amino acid tryptophan (Trp) into downstream catabolites known as kynurenines. IDO1 can decrease the tryptophan and produce a series of toxic kynurenine metabolites to promote the immune toleration via GCN2 pathway, mTOR pathway, toxic effect of kynurenine and favoring differentiation of Tregs. The depletion of Trp, and the accumulation of Kyn have been proposed as mechanisms that contribute to the suppression of the immune response-primarily evidenced by in vitro study. IDO1 is therefore considered to be an immunosuppressive modulator and quantification of IDO1 metabolism may be critical to understanding its role in select immunopathologies, including autoimmune- and oncological-conditions, as well as for determining the potency of IDO1 enzyme inhibitors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:1.13.11.52, IDO, IDO 1, IDO-1, INDO |