Recombinant Human HSP27 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4620
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human HSP27 protein Ala70-Thr184 (Accession# P04792) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 3315 |
| Accession | P04792 |
| PredictedSize | 39.0 kDa |
| SDS-PAGE | 40-55 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Heat shock protein 27 (HSP27), also known as HSPB1, belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSP27 has been found to be a valuable marker for melanoma.
References:
1. Kijima K. et al. (2005). J Hum Genet. 50(9):473-476. 2. Foster CS. et al.(2009). Br J Cancer. 101(7):1137-44. 3. Okuno M. et al. (2016). Int J Mol Sci. 17(1):137. 4. Seo MK. et al. (2020). Pharmacoecon Open. 4(3):529-539.