Human HMGB2 ELISA Kit
Sensitivity
0.18 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Cat no : KE00558
Synonyms
high mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2, HMG2
Validation Data Gallery
Product Information
KE00558 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HMGB2 ELISA kit is to be used to detect and quantify protein levels of endogenous human HMGB2. The assay recognizes human HMGB2. An antibody specific for human HMGB2 has been pre-coated onto the microwells. The human HMGB2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HMGB2 is added to detect the captured human HMGB2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HMGB2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.18 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3148 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 76% | 73%-80% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 8.08 | 0.29 | 3.54 |
| 2 | 8 | 3.30 | 0.15 | 4.56 |
| 3 | 8 | 2.55 | 0.10 | 4.03 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 11.94 | 0.31 | 2.62 |
| 2 | 16 | 3.47 | 0.27 | 7.82 |
| 3 | 16 | 1.64 | 0.18 | 10.94 |
Background Information
High mobility group protein B2 (HMGB2) belongs to a family of highly conserved proteins that contain HMG box domains. All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-terminal acidic domain. HMGB1 is a widely expressed and highly abundant protein. HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals. HMGB3 is only expressed during embryogenesis. While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of DNA, which results in local bending of the DNA double helix . HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA binding proteins to their cognate binding sites in chromatin. For example, HMGB1 and HMGB2 facilitate the binding of Hox proteins, Oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters . Furthermore, HMGB2 interacts with RAG1 to facilitate RAG complex binding to the recombinant signal sequence (RSS) and stimulate DNA-bending and subsequent VDJ cleavage at antigen receptor genes. In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB2 is secreted by myeloid cells and promotes proliferation and migration of endothelial cells by binding to the receptor for advanced glycation endproducts (RAGE). Research studies have shown that HMGB2 overexpression in hepatocellular carcinoma is associated with poor prognosis and shorter survival time.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | high mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2, HMG2 |