Speedy™ Human HER2/ErbB2 One-Step ELISA Kit
Sensitivity
7.8 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Cat no : SE50015
Synonyms
ERBB2, CD340, HER 2, HER 2/neu, HER2
Validation Data Gallery
Product Information
| Product name | Speedy™ Human HER2/ErbB2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Human milk, Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 7.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2064 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 102% | 82%-117% |
| Cell culture supernatant | 110% | 103%-117% |
| Urine | 93% | 76%-118% |
| Human milk | 111% | 105%-117% |
| Lysate | 101% | 94%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,920.4 | 25.6 | 1.3 |
| 2 | 8 | 559.1 | 9.4 | 1.7 |
| 3 | 8 | 331.0 | 28.5 | 8.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,912.2 | 35.7 | 1.9 |
| 2 | 16 | 558.8 | 9.6 | 1.7 |
| 3 | 16 | 327.0 | 27.8 | 8.5 |
Background Information
The human epidermal growth factor receptor (HER) family of receptors plays a central role in the pathogenesis of several human cancers. HER2 (human epidermal growth factor receptor 2), also known as ErbB2 and Neu, is 1 of the 4 membrane receptor tyrosine kinases (RTKs) and when activated affects cell proliferation and survival. HER2 is expressed in many tissues and its major role in these tissues is to facilitate excessive/uncontrolled cell growth and tumorigenesis. Additionally, HER2 mutations have also been reported in common cancers such as pulmonary, colorectal, and breast cancers, indicating a potential for HER2-directed therapies in these cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ERBB2, CD340, HER 2, HER 2/neu, HER2 |