Speedy™ Human Growth Hormone One-Step ELISA Kit
Sensitivity
0.5 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : SE50216
Synonyms
GH, GH1, GH 1, GH-N, growth hormone 1
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Growth Hormone One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2688 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 93% | 85%-106% |
| Cell culture supernatant | 115% | 102%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 507.9 | 10.2 | 2.0 |
| 2 | 8 | 139.9 | 13.9 | 9.9 |
| 3 | 8 | 69.4 | 3.5 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 510.8 | 9.7 | 1.9 |
| 2 | 16 | 134.0 | 11.4 | 8.5 |
| 3 | 16 | 67.4 | 3.6 | 5.3 |
Background Information
Growth hormone (GH) is a heterogeneous polypeptide consisting of a number of different isoforms and variants. Its secretion from pituitary somatotrophs is pulatile, with low or undetectable basal levels between peaks. GH secretion has a characteristic diurnal pattern with maximal levels recorded during sleep. Growth hormone is a powerful anabolic hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. The actions of GH are mediated by GH receptors (GHR), which are widely distributed among tissues. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GH, GH1, GH 1, GH-N, growth hormone 1 |