Human Granzyme B ELISA Kit1 Publications

カタログ番号: KE00121

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human Granzyme B ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
2.6 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 85% 77%-95%
Cell culture supernatants 91% 77%-99%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 26.6 1.0 3.8
2 20 112.0 5.2 4.6
3 20 465.7 31.7 6.8

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 27.4 2.7 9.7
2 24 119.4 7.2 6.0
3 24 499.8 27.7 5.5

製品概要
KE00121 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Granzyme B ELISA kit is to be used to detect and quantify protein levels of endogenous Granzyme B. The assay recognizes human Granzyme B. An antibody specific for Granzyme B has been pre-coated onto the microwells. The Granzyme B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Granzyme B is added to detect the captured Granzyme B protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA1, CTSGL1, Endogenous granzyme B, GZMB, HLP, Homo sapiens (Human), SECT
背景説明

Granzyme B (GZMB) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease Granzyme B (GZMB) can promote apoptosis through direct processing and activation of members of the caspase family. Granzyme B also functions as processing protease of cytokines, including interleukin (IL)-18 and IL-1a, and extracellular matrix proteins, including fibronectin and various proteoglycans. Plasma Granzyme B concentrations were significantly higher in patients with Atopic dermatitis (AD) and psoriasis than in healthy controls, which indicated that Granzyme B may play an important role in the induction of intractable itch and dermatitis in AD.


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