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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Human Granzyme B protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0592

Validation Data Gallery

  • Purity of Recombinant Human Granzyme B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Granzyme B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression CHO-derived Human Granzyme B protein Gly19-Tyr247 (Accession# P10144) with a His tag at the C-terminus.
GeneID 3002
Accession P10144
PredictedSize 27.4 kDa
SDS-PAGE 30-35 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 300 mM NaCl, pH 7.4. Normally 2.5% trehalose and 2.5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization. GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA. This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei. GzmB also forms dimers.

References:

1. Cullen, Sean P et al. The Journal of cell biology vol. 176,4 (2007): 435-44.
2. Zhang, D et al. Proceedings of the National Academy of Sciences of the United States of America vol. 98,10 (2001): 5746-51.
3. Trapani, J A et al. The Journal of biological chemistry vol. 271,8 (1996): 4127-33.