Recombinant Human Granzyme A protein (mFc Tag) (HPLC verified)
Species
Human
Purity
>90 %, SDS-PAGE
>90%, SEC-HPLC
Tag
mFc Tag
Activity
not tested
Cat no : Eg3925
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90%, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Granzyme A protein Glu27-Val262 (Accession# P12544-1) with a mouse IgG Fc tag at the C-terminus. |
| GeneID | 3001 |
| Accession | P12544-1 |
| PredictedSize | 52.7 kDa |
| SDS-PAGE | 50-60 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Granzyme A (GzmA) is a serine protease primarily expressed by cytotoxic T lymphocytes and natural killer (NK) cells, playing a critical role in immune-mediated cytotoxicity. It is involved in the elimination of target cells, such as virus-infected cells or tumor cells, by inducing apoptosis. GzmA is associated with various diseases, particularly those involving immune responses and inflammation. It plays a significant role in the pathogenesis of sepsis, where its inhibition reduces inflammation and increases survival.
References:
1.Zhou Z. et al. (2020). Science. 368(6494):eaaz7548. 2.Hay ZLZ. et al. (2022). Int J Mol Sci. 23(3):1833. 3.Garzón-Tituaña M. et al. (2021). Theranostics. 11(8):3781-3795.