KE00087 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GMFG ELISA kit is to be used to detect and quantify protein levels of endogenous GMFG. The assay recognizes human GMFG. A polyclonal antibody specific for GMFG has been pre-coated onto the microwells. The GMFG protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for GMFG is added to detect the captured GMFG protein. For signal development,horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent.Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.