Recombinant Human GITR/TNFRSF18 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg1356
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human GITR/TNFRSF18 protein Gln26-Glu161 (Accession# Q9Y5U5-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 8784 |
| Accession | Q9Y5U5-1 |
| PredictedSize | 40.8 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
GITR is a type I transmembrane protein that contains a cysteine-rich extracellular domain and an intracellular domain that is highly homologous to TNFR family members 4-1BB/CD137 and CD27. GITR is expressed on a variety of immune cells, including regulatory T cells (Tregs), effector T cells (Teffs), natural killer cells (NK cells), B cells, and certain myeloid cells. Activation of GITR can promote the activation, proliferation, and cytokine secretion of effector T cells.
References:
1. Oja, Anna E et al. Immunology letters vol. 222 (2020): 73-79. 2. Wang, Feng et al. Nature communications vol. 12,1 (2021): 1378. 3. Ming, Siqi et al. Frontiers in immunology vol. 12 (2021): 736269. 4. Chan, Sarah et al. Nature cancer vol. 3,3 (2022): 337-354.