Recombinant Human TNFSF18 protein (hFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
hFc Tag
Activity
not tested
Cat no : Eg0067
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human TNFSF18 protein Gln50-Ser177 (Accession# Q9UNG2) with a human IgG1 Fc tag at the N-terminus. |
| GeneID | 8995 |
| Accession | Q9UNG2 |
| PredictedSize | 40.9 kDa |
| SDS-PAGE | 40-48 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
TNFSF18 is also named as Glucocorticoid-induced TNF-related ligand (hGITRL). GITR is a gene coding for a member of the TNF receptor superfamily. GITR activation by its ligand (GITRL) influences the activity of effector and regulatory T cells, thus participating in the development of immune response against tumours and infectious agents, as well as in autoimmune and inflammatory diseases. GITR-GITRL interactions co-stimulate both responder T-cell functions and the suppressive functions of TReg cells. GITRL is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
References:
1. Giuseppe Nocentini. et al. (2007) Eur J Immunol. 37(5):1165-9. 2. Ethan M Shevach. et al. (2006) Nat Rev Immunol. 6(8):613-8. 3. Pedro M L. et al. (2013) J Pharmacol Exp Ther. 347(1):164-72.