Human GILZ ELISA Kit
Sensitivity
0.15 ng/mL
Range
0.94-60 ng/mL
Reactivity
Human
Cat no : KE00768
Synonyms
Delta sleep-inducing peptide immunoreactor, DSIPI, DSIP-immunoreactive peptide, TSC 22R, TSC22 D3
Validation Data Gallery
Product Information
KE00768 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GILZ ELISA kit is to be used to detect and quantify protein levels of endogenous human GILZ. The assay recognizes human GILZ. An antibody specific for human GILZ has been pre-coated onto the microwells. The human GILZ protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GILZ is added to detect the captured human GILZ protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GILZ ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.15 ng/mL |
| Range | 0.94-60 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1831 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 82% | 79%-84% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 28.61 | 0.82 | 2.86 |
| 2 | 8 | 7.86 | 0.29 | 3.69 |
| 3 | 8 | 4.05 | 0.22 | 5.44 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 28.74 | 0.66 | 2.30 |
| 2 | 16 | 7.66 | 0.32 | 4.22 |
| 3 | 16 | 3.89 | 0.28 | 7.22 |
Background Information
Glucocorticoid-induced leucine zipper (GILZ) is a dexamethasone-inducible gene belonging to the TSC-22 family of proteins, which are characterized by the presence of TSC box and leucine zipper domains. GILZ expression is rapidly and ubiquitously induced by glucocorticoids (GCs) in various types of cells, including lymphoid cells, in which it regulates activation and apoptosis. An emerging body of literature has established GILZ as an important mediator of GC anti-inflammatory effects in cell lineages and in inflammatory mouse models, by inhibiting expression of pro-inflammatory genes. GILZ has also been found to be involved in allergy, being downregulated in DCs of respiratory allergic patients or in the airways of allergen-challenged asthmatic subjects and upregulated after GC treatment.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Delta sleep-inducing peptide immunoreactor, DSIPI, DSIP-immunoreactive peptide, TSC 22R, TSC22 D3 |