Speedy™ Human GDF9 One-Step ELISA Kit
Sensitivity
0.14 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human
Cat no : SE50142
Synonyms
GDF 9, GDF-9
Validation Data Gallery
Product Information
| Product name | Speedy™ Human GDF9 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.14 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2661 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 84% | 73%-99% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 27.77 | 0.46 | 1.66 |
| 2 | 8 | 6.85 | 0.17 | 2.48 |
| 3 | 8 | 3.49 | 0.08 | 2.29 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 27.49 | 0.58 | 2.11 |
| 2 | 16 | 6.86 | 0.15 | 2.19 |
| 3 | 16 | 3.49 | 0.09 | 2.58 |
Background Information
GDF9 (Growth/differentiation factor 9) belongs to the TGF-beta family. GDF9 is required for ovarian folliculogenesis. GDF9 are oocyte-secreted factors with a leading role in the control of ovarian function in female reproduction, modulating both the cell fate of the somatic granulosa cells and the quality and developmental competence of the egg. GDF9 promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. GDF9 attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GDF 9, GDF-9 |