Human GDF9 ELISA Kit
Sensitivity
0.42 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human
Cat no : KE00502
Synonyms
GDF9,GDF 9,GDF-9
Validation Data Gallery
Product Information
KE00502 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GDF9 ELISA kit is to be used to detect and quantify protein levels of endogenous human GDF9. The assay recognizes human GDF9. An antibody specific for human GDF9 has been pre-coated onto the microwells. The human GDF9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GDF9 is added to detect the captured human GDF9 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GDF9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.42 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2661 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 86% | 83%-90% |
| Cell lysate | 83% | 70%-95% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 23.85 | 1.30 | 5.44 |
| 2 | 8 | 6.12 | 0.10 | 1.71 |
| 3 | 8 | 3.30 | 0.19 | 5.84 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 23.87 | 1.17 | 4.88 |
| 2 | 16 | 6.05 | 0.25 | 4.10 |
| 3 | 16 | 3.37 | 0.19 | 5.61 |
Background Information
GDF9 (Growth/differentiation factor 9) belongs to the TGF-beta family. GDF9 is required for ovarian folliculogenesis. GDF9 are oocyte-secreted factors with a leading role in the control of ovarian function in female reproduction, modulating both the cell fate of the somatic granulosa cells and the quality and developmental competence of the egg. GDF9 promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. GDF9 attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GDF9,GDF 9,GDF-9 |