Human/Mouse/Rat GDF-8 ELISA Kit

Sensitivity

2.3 pg/mL

Range

15.6-1000 pg/mL, 31.25-2000 pg/mL

Reactivity

Human/Mouse/Rat

Cat no : KE00120

Synonyms

GDF 8, GDF8, MSTN, myostatin



Product Information

KE00120 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GDF-8 ELISA kit is to be used to detect and quantify protein levels of endogenous GDF-8. The assay recognizes human,mouse and Rat GDF-8. An antibody specific for GDF-8 has been pre-coated onto the microwells. The GDF-8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GDF-8 is added to detect the captured GDF-8 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human/Mouse/Rat GDF-8 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Tissue lysates
Assay type Sandwich
Sensitivity 2.3 pg/mL
Range 15.6-1000 pg/mL, 31.25-2000 pg/mL
Reactivity Human/Mouse/Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 2660

Recovery

Sample TypeAverageRange
Mouse serum113%107%-121%
Rat serum95%75%-117%
Cell culture supernatants88%71%-109%
tissue lysates93%81%-111%
Human plasma111%90%-123%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12052.01.52.8
220212.47.23.4
320877.128.33.2

InterAssay

Samplenmean ( pg/mL)SDCV%
12454.13.05.5
224217.96.63.0
324854.327.43.2

Background Information

Growth Differentiation Factor 8 (GDF-8), also called myostatin, is a member of the transforming growth factor (TGF)-βsuper-family. GDF-8 is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. GDF-8 is also expressed in the human reproductive system, such as in granulosa cells, follicular fluid and trophoblasts. In addition, GDF-8 has also functions in heart and adipose tissue, and is related to interstitial fibrosis in the heart, human cancer cachexia, and activation of inflammatory cytokines and insulin resistance.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms GDF 8, GDF8, MSTN, myostatin

Publications

SpeciesSample TypeTitle
mouseserum

Br J Pharmacol

A new therapeutic effect of fenofibrate in Duchenne muscular dystrophy: The promotion of myostatin degradation.

Authors - Zeren Sun
humanserum

Nutrition

Viral eradication by direct-acting antivirals does not decrease the serum myostatin level in patients infected with hepatitis C virus.

Authors - Kei Endo
humanfollicular fluid

Integr Med Res

Chinese herbal medicine (Bu-Shen-Tian-Jing Formula) for outcomes of IVF in Chinese patients with polycystic ovary syndrome: a retrospective cohort study.

Authors - Xiaoming Pan