Human/Mouse/Rat GDF-8 ELISA Kit0 Publications

カタログ番号: KE00120

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human/Mouse/Rat GDF-8 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants, Tissue lysates

アッセイ方法:
Sandwich

感度:
2.3 pg/mL

測定範囲:
15.6-1000 pg/mL, 31.25-2000 pg/mL

測定可能動物種:
Human/Mouse/Rat

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 113% 107%-121%
Rat serum 95% 75%-117%
Cell culture supernatants 88% 71%-109%
tissue lysates 93% 81%-111%
Human plasma 111% 90%-123%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 52.0 1.5 2.8
2 20 212.4 7.2 3.4
3 20 877.1 28.3 3.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 54.1 3.0 5.5
2 24 217.9 6.6 3.0
3 24 854.3 27.4 3.2

製品概要
KE00120 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GDF-8 ELISA kit is to be used to detect and quantify protein levels of endogenous GDF-8. The assay recognizes human,mouse and Rat GDF-8. An antibody specific for GDF-8 has been pre-coated onto the microwells. The GDF-8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GDF-8 is added to detect the captured GDF-8 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
GDF 8, GDF8, MSTN, myostatin
背景説明

Growth Differentiation Factor 8 (GDF-8), also called myostatin, is a member of the transforming growth factor (TGF)-βsuper-family. GDF-8 is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. GDF-8 is also expressed in the human reproductive system, such as in granulosa cells, follicular fluid and trophoblasts. In addition, GDF-8 has also functions in heart and adipose tissue, and is related to interstitial fibrosis in the heart, human cancer cachexia, and activation of inflammatory cytokines and insulin resistance.


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