Human FMO1 ELISA Kit
Sensitivity
0.12 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human
Cat no : KE00521
Synonyms
FMO1,Dimethylaniline monooxygenase [N-oxide-formin
Validation Data Gallery
Product Information
KE00521 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FMO1 ELISA kit is to be used to detect and quantify protein levels of endogenous human FMO1. The assay recognizes human FMO1. An antibody specific for human FMO1 has been pre-coated onto the microwells. The human FMO1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FMO1 is added to detect the captured human FMO1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FMO1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.12 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2326 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 90% | 80%-102% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 23.29 | 1.29 | 5.54 |
| 2 | 8 | 5.03 | 0.15 | 3.08 |
| 3 | 8 | 1.24 | 0.10 | 8.31 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 21.65 | 2.33 | 10.77 |
| 2 | 16 | 4.62 | 0.48 | 10.37 |
| 3 | 16 | 1.19 | 0.11 | 9.37 |
Background Information
Flavin-containing monooxygenase 1 (FMO1) belongs to the FMO family. FMO1 is an enzyme that catalyzes the oxygenation of a series of sulfur-containing and nitrogen-containing substrates, and thus is involved in a series of drugs and xenobiotics metabolism. FMO1 can regulate PPAR-α and mediate metabolic processes. FMO1 is expressed at relatively high levels in human fetal liver and also represents the major FMO enzyme in the adult human and rabbit intestine and kidney.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FMO1,Dimethylaniline monooxygenase [N-oxide-formin |