Human FGF2 ELISA Kit0 Publications

カタログ番号: KE00129

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human FGF2 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Cell lysates

アッセイ方法:
Sandwich

感度:
18.8 pg/mL

測定範囲:
31.25-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell lysates 95% 81%-124%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 507.6 25.6 5.0
2 20 110.7 6.3 5.7
3 20 62.3 3.0 4.8

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 500.7 21.9 4.4
2 24 114.3 9.8 8.6
3 24 63.3 3.1 4.9

製品概要
KE00129 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FGF2 ELISA kit is to be used to detect and quantify protein levels of endogenous FGF2. The assay recognizes human FGF2. An antibody specific for FGF2 has been pre-coated onto the microwells. The FGF2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for FGF2 is added to detect the captured FGF2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Basic fibroblast growth factor, BFGF, FGF2, FGFB, HBGF 2
背景説明

Fibroblast growth factor 2 (FGF2) is one of the most well-studied members of the fibroblast growth factor superfamil. FGF2 can be synthesized and secreted by human adipocytes. FGF2 activates its target receptor tyrosine kinases, the FGFRs, on the cell surface in order to activate numerous downstream pathways, including several mitogen activated protein kinase (MAPK) pathways. FGF2 inhibited TGFβ-mediated fibroblast activation, resulting in more rapidly proliferating, spindle-shaped cells, compared to the more slowly proliferating, flatter TGFβ-treated cells. FGF2 is an important regulator of cell growth and differentiation under physiological and pathological conditions. FGF2 is widely involved in important biological processes such as stem cell proliferation and angiogenesis.


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