Human FGF2 ELISA Kit

Sensitivity

18.8 pg/mL

Range

31.25-1000 pg/mL

Reactivity

Human

Cat no : KE00129

Synonyms

Basic fibroblast growth factor, BFGF, FGF2, FGFB, HBGF 2



Product Information

KE00129 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FGF2 ELISA kit is to be used to detect and quantify protein levels of endogenous FGF2. The assay recognizes human FGF2. An antibody specific for FGF2 has been pre-coated onto the microwells. The FGF2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for FGF2 is added to detect the captured FGF2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human FGF2 ELISA Kit
Tests 1 X 96 well plate
Sample type Cell lysates
Assay type Sandwich
Sensitivity 18.8 pg/mL
Range 31.25-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 2247

Recovery

Sample TypeAverageRange
Cell lysates95%81%-124%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120507.625.65.0
220110.76.35.7
32062.33.04.8

InterAssay

Samplenmean ( pg/mL)SDCV%
124500.721.94.4
224114.39.88.6
32463.33.14.9

Background Information

Fibroblast growth factor 2 (FGF2) is one of the most well-studied members of the fibroblast growth factor superfamil. FGF2 can be synthesized and secreted by human adipocytes. FGF2 activates its target receptor tyrosine kinases, the FGFRs, on the cell surface in order to activate numerous downstream pathways, including several mitogen activated protein kinase (MAPK) pathways. FGF2 inhibited TGFβ-mediated fibroblast activation, resulting in more rapidly proliferating, spindle-shaped cells, compared to the more slowly proliferating, flatter TGFβ-treated cells. FGF2 is an important regulator of cell growth and differentiation under physiological and pathological conditions. FGF2 is widely involved in important biological processes such as stem cell proliferation and angiogenesis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Basic fibroblast growth factor, BFGF, FGF2, FGFB, HBGF 2

Publications

SpeciesSample TypeTitle
humancell lysates

Food Res Int

Efficient expression of a cytokine combination in Saccharomyces cerevisiae for cultured meat production

Authors - Qingzi Lei
chickencell lysates

Biotechnol J

Evaluation of expression systems for recombinant protein production in chicken egg bioreactors

Authors - Ryota Kajihara
human

J Dent

Calvaria defect regeneration via human periodontal ligament stem cells and prevascularized scaffolds in athymic rats

Authors - Zeqing Zhao
humancell culture supernatants

ACS Nano

Controllably Self-Assembled Antibacterial Nanofibrils Based on Insect Cuticle Protein for Infectious Wound Healing

Authors - Qiuyu Gong