β-Estradiol ELISA Kit
Sensitivity
0.3 pg/mL
Range
46.88-3000 pg/mL
Cat no : KE30010
Synonyms
17β-Estradiol
Validation Data Gallery
Product Information
KE30010 is a vitro competitive binding enzyme-linked immunosorbent assay(Competitive ELISA). The β-Estradiol ELISA kit is to be used to detect and quantify protein levels of vitro human β-Estradiol. The assay recognizes human β-Estradiol. A 96-well plate has been precoated with β-Estradiol antigen. Samples and the antibody-Bio conjugate are added to the wells, where β-Estradiol in the sample competes with the β-Estradiol antigen immobilized on the microplate for binding with the antibody-Bio. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of β-Estradioll in the sample and the intensity is measured at 450 nm with the correction wavelength set at 630 nm.
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 84%-128% |
| Urine | 99% | 93%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,550.9 | 68.9 | 4.4 |
| 2 | 8 | 394.1 | 34.4 | 8.7 |
| 3 | 8 | 191.2 | 16.0 | 8.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,539.4 | 72.7 | 4.7 |
| 2 | 16 | 391.1 | 36.5 | 9.3 |
| 3 | 16 | 176.6 | 24.9 | 14.1 |
Background Information
β-Estradiol is the primary and most potent estrogen sex hormone in the human body, particularly in non-pregnant females of reproductive age. It is a steroid hormone essential for the development and regulation of the female reproductive system, secondary sexual characteristics, and numerous other physiological processes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 17β-Estradiol |