Speedy™ Human ErbB3/HER3 One-Step ELISA Kit
Sensitivity
5.6 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Cat no : SE50076
Synonyms
ERBB3, c erbB 3, c erbB3, EC:2.7.10.1, ErbB 3
Validation Data Gallery
Product Information
| Product name | Speedy™ Human ErbB3/HER3 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva, Human milk, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 5.6 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2065 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 106% | 95%-113% |
| Cell culture supernatant | 111% | 107%-119% |
| Urine | 113% | 109%-120% |
| Saliva | 97% | 87%-104% |
| Human milk | 95% | 90%-101% |
| Cell lysate | 83% | 77%-91% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,185.8 | 131.7 | 6.0 |
| 2 | 8 | 534.1 | 7.5 | 1.4 |
| 3 | 8 | 268.0 | 10.4 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,166.8 | 93.9 | 4.3 |
| 2 | 16 | 539.5 | 19.7 | 3.7 |
| 3 | 16 | 274.7 | 19.9 | 7.2 |
Background Information
ErbB3/HER3 is a member of the ErbB family of receptor tyrosine kinases, which play crucial roles in cell proliferation, survival, and differentiation. ErbB3/HER3 is a type I transmembrane glycoprotein and is unique within the ErbB family because it lacks intrinsic kinase activity. Instead, it relies on forming heterodimers with other ErbB receptors, particularly HER2, to mediate signaling. HER3 has emerged as a key player in resistance to other HER family-targeted therapies, such as tyrosine kinase inhibitors and monoclonal antibodies against EGFR or HER2.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ERBB3, c erbB 3, c erbB3, EC:2.7.10.1, ErbB 3 |